Hepatitis C Viral Diagnostic Kit(Fluorescence PCR)

【Principle】

This test kit applies magnetic bead method to extract HCV RNA from serum or plasma specimens.  It uses a pair of specific primers and a specific fluorescent probe (TaqMan probe 5 ‘ end labeled FAM fluorescein) designed for the conserved region of HCV nucleic acid, matched with RT-PCR reaction solution, using the Real-time PCR detection technology  to realize the quantitative detection of HCV RNA through the change of fluorescence signals on the fluorescence quantitative PCR instrument.

【Intended use】

This test kit is used for quantitative detection of hepatitis C virus (HCV) RNA in clinical serum or plasma specimens. The test results can be used for auxiliary diagnosis of hepatitis C and observation of antiviral drug efficacy. It is beneficial for the early diagnosis of hepatitis C and provides reference for guiding medication.

【Characteristics】

【Advantages】

● Extraction of nucleic acid by magnetic bead method, simple operation steps and high nucleic acid extraction efficiency;

● The specific RT-PCR amplification reaction system has strong anti-interference ability. Test results not affected by specimens containing hemolysis, hyperbilirubin, hyperlipidemia and common antiviral drugs;

● High sensitivity, accurate quantification and good repeatability, both inter-assay and intra-assay coefficient of variation (CV)  ≤ 5%;

● High efficiency internal standard technology to avoid false negative test results.